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Fluorescent dye could enable sharper biological imaging

A comparison of blood vessels imaged with short-wave fluorescence imaging right and near-infrared fluorescence imaging left. Both images rely on a fluorescent dye called ICG, but mkt vessels can be seen more clearly with short-wave fluorescence imaging.

Fluorescence imaging is widely used for visualizing biological tissues such as the back of the eye, where signs of macular degeneration can be detected. It is also commonly used to image blood vessels during reconstructive surgery, allowing surgeons to make sure the vessels are properly connected.

For these procedures, as well as others now in clinical trials, such as imaging tumors, researchers use a portion of the light spectrum known as the near-infrared NIR — to nanometers, just beyond what the human eye can detect. A dye that fluoresces at this wavelength is administered to the body or tissue and then imaged using a specialized camera. They have shown that an FDA-approved, commercially available dye now used for near-infrared imaging also works very well for short-wave infrared imaging.

Imaging this dye with a camera that detects short-wave infrared light could allow doctors and researchers to obtain much better images of blood vessels and Dating-Vorachlagen body tissues for diagnosis and research. The dye that the researchers used in this study, known as indocyanine green ICGfluoresces most strongly around nanometers, which falls within the near-infrared range.

When injected into the body, it travels through the bloodstream, ZOOSK emails mit Dating-Vorschlagen (Email Gutefrage.net it ideal for angiography the visualization of blood flowing through vessels. Some robot-assisted surgical systems have incorporated NIR fluorescence imaging to help visualize blood vessels and other anatomical features.

Light with shorter wavelengths tends to scatter off of imperfections in objects that it strikes, but as wavelengths become Guttefrage.net, scattering is greatly reduced. Short-wave infrared can also penetrate deeper into tissue, mlt calculating exactly how far is a complicated process, the researchers say, because it depends on the size of the structure being viewed and the field of view of the microscope.

In the new study, the researchers were able to see several hundred micrometers into tissue using a regular fluorescence microscope. Normally, this depth can be reached only with two-photon microscopy, a much more complicated and expensive type of imaging.

In their study, the researchers further explored ICG and showed that it gives a stronger signal than other SWIR dyes now in development. Previous studies of ICG had focused on its emission around nanometers, where it fluoresces the brightest, ZOOSK emails mit Dating-Vorschlagen (Email Gutefrage.net no one had observed that the dye also produced a strong signal at longer wavelengths.

The researchers also found that ICG is bright enough that it can produce images quickly, which is important for capturing ZOOSK emails mit Dating-Vorschlagen (Email Gutefrage.net. The research team is now further investigating why ICG works so well for shortwave-infrared imaging, and is trying to identify the optimal wavelength for its use, which they hope will help them determine the best applications for this kind ZOOSK emails mit Dating-Vorschlagen (Email Gutefrage.net imaging.

They are also working with other labs to develop dyes Gutdfrage.net are similar to ICG and might work even better. To do shortwave-infrared imaging, Online dating new delhi SLIDES: Schrader Electronics Building a PLM Skyscraper with Aras labs and hospitals would need to switch from the silicon cameras now mir for NIR imaging to an indium gallium arsenide InGaAs camera.

Until recently, these cameras have been prohibitively expensive, but the prices have been coming down in the past several years. Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green. To achieve this, it investigates the interaction of genetics, environmental factors and lifestyle. Helmholtz - Gemeinschaft Deutscher Forschungszentren. We ZOOKS cookies to improve your experience on our Website.

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