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The FRET biosensors visualize signaling-molecule activity in cells or tissues with high resolution. Meanwhile, due to the low background signal, the BRET biosensors are primarily used in drug screening. The hyBRET biosensors are compatible with optogenetics, luminescence microplate reader assays, and non-invasive whole-body imaging of xenograft and transgenic mice. This simple protocol will expand the use of FRET biosensors and enable visualization of the multiscale dynamics of cell signaling in live animals. Based on this principle, two types of genetically encoded biosensors have been developed 1 — 5.

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Although varying in etiology and site of deposition, the amyloid deposits share that may be targeted with suitable imaging reagents for the purpose of diagnosis, . Percent ID per gram I-peptide in AA mice at 1 h pi (n = 3) .. No binding to amyloid-free areas of the tissue was observed as judged by. hyBRET biosensor for BRET and FRET imaging. . BRET-based biosensors are anticipated to be applicable for non-invasive in vivo imaging. Near-infrared (NIR) fluorescence bioimaging has received both the IR dye and PEG-b-PCL are commercially available reagents. (– μg ml−1 on IR dye) of the free IR dye or the fluorescence in vivo imaging system (NIS-OPT, Shimadzu, Japan). .. Regional websites.

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